SLU-PP-332 reconstitution woes!

Hong Kong Yanpep International
Witty-IMO

Witty-IMO

Active member
Hey all, I'm in a bind. I got some SLU-PP-332 to inject, but didn't realize how tricky it is to mix. It seems like just using bacteriostatic water is a no-go because it's so hydrophobic.

I saw some videos suggesting using 0.5ml of DMSO followed by the same amount of BAC water. But everything I'm reading says injecting DMSO is super toxic at that concentration. I also found some info on using DMSO, then polysorbate 80, then buffered saline to dilute the DMSO to around 1%. Anyone have experience with this? I'm open to suggestions – and yes, feel free to point out my mistake, LOL.
 
Okay, so nobody else has tried this either, haha. Well, I kept digging, and found this:

Apparently, to dissolve really hydrophobic peptides, you can use a tiny bit of DMSO (like 30-50 μl at 100%), and then slowly drip that into a stirring buffered solution like PBS. They said if it gets cloudy, you've used too much peptide. Sonication may also assist.

They also had this handy rule of thumb:

* 0.1% DMSO: Usually safe for cells.
* 0.5% DMSO: Commonly used in cell culture without problems.
* 1% DMSO: Okay for some cells, but 0.5% is better.
* 5% DMSO: Has been used successfully in some cells.

Still researching...
 
That's precisely why I chose capsules. I'm unsure if they are more effective or not, but they definitely offer superior convenience.
 
I've seen people mention using 0.2mL or 0.3mL of DMSO with 2.3mL or 2.2mL of BAC water and claiming decent results. Maybe diluting the DMSO with more BAC could help?
 
Reconstituting peptides can certainly be tricky. While DMSO can be used, it's crucial to minimize exposure. The suggestions of using a small amount of DMSO followed by a buffer like PBS are reasonable. Ensure the final DMSO concentration is as low as possible, ideally below 0.5%, to minimize potential toxicity. Also, remember sterile technique is paramount.
 
Concerning pre-reconstituted peptides, I'd advise caution. I saw a discussion where someone received ipamorelin/cjc-1295 already mixed. It's best to inquire about shipping conditions, specifically if it was shipped cold. The stability of peptides in solution is significantly affected by temperature. If unsure, it's generally safer to reconstitute yourself. It sure would be nice to have a reference guide for proper handling of different peptides.
 
I've been wondering about the best reconstitution liquid. I've seen some mentions of acetic acid instead of bacteriostatic water, supposedly for longer shelf life, specifically for IGF peptides. Has anyone looked into this?
 
the NaCl concern is worth noting before anyone goes that route based on availability - standard saline is not the same as BAC water or sterile water for injection, and the ionic interaction with the lyophilization buffer is a real mechanism not just a precaution. sterile water or BAC water is the right starting point for most compounds
 
Ampuwa is a reliable choice for reconstitution - the glass bottle format prevents the leaching issues some people report with plastic-sealed sterile water, and the 50ml volume per bottle is practical for working stock that gets used quickly. The pharmaceutical-grade sterile water standard it meets is the benchmark worth looking for in alternatives too.
 
The only way a genuinely clear mixture came together was combining equal parts of two solvents, one first, then slowly adding the second. Burned through most of a half-dozen vials just getting there through trial and error.

Now genuinely curious why the earliest batches mixed clean using just the standard diluent alone.

Edit: after reading more about injecting that particular solvent combination, abandoning this whole approach entirely.
 
I've exhausted every method I can think of for mixing slu-pp 332. It goes clear, then 15-20 minutes later it snow globes. My mix of dmso, peg 400 and hospira simply collapses. Reading says injected dmso should stay at or under 10%, because more irritates skin or kills cells. A 10% version means .5ml dmso, 2.2ml hospira and 30 units peg 400. Using less bac keeps it stable, though then the dmso share climbs too high.
 
My aim is a repeatable mix for slu-pp-332, and burning a few vials while experimenting is acceptable, I'm mostly enjoying the tinkering. Following what others tried, I did a .5ml DMSO recon then added it to bac water. Right after mixing it looked perfectly clear, yet by the following morning a milky film sat on the vial floor. A gentle swirl of the vial and the haze appeared to melt back into the liquid. Almost 2 days later I rechecked and it's stable, the cloudiness hasn't returned and nothing floats in it. Any thoughts?
 
I've tried reconstituting several vials and always get a cloudy mess. I put 2mL bac water in a sterile vial, dissolved the SLU in 0.5mL DMSO, then added the DMSO very slowly, a drop each time with roughly 20 seconds and gentle swirling in between, into the 2mL bac vial using the reverse titration method, and no luck. Wasted five vials that way, and heaps of time.
 
I've reconstituted plenty of vials without trouble by using DMSO first and bac water after, with hospira as my only diluent to avoid issues. Add 0.5ml DMSO very gradually, one drop after another, and let the powder dissolve completely. Then slowly pour in 2ml bac while gently swirling the vial. For a 5mg vial that lands at 2mg per 1ml. It lives in my fridge, dark, and remains crystal clear. The one peptide that gave me trouble was kisspeptin, two vials gelled at once with hospira. Adding AA cleared one, but I gave up on them, not worth the risk. Probably my mistake, but I started trt so it's pointless for me now.
 
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