Reconstituting peps - noob q's

Bug_Gal

Bug_Gal

Well-known member
Hey everyone, I've been searching but can't find the answers I need, so any help would be great! I'm gonna be doing my first reconstitution soon, and just want to make sure I don't screw anything up lol.

1. Should the bacteriostatic water and the peptides be the same temperature before I mix them? If the water was in the fridge or the peps were in the freezer, how long should I let them sit out to warm up?

2. I have small 3mL vials. The person who used to do this for me just used the little insulin needles that I inject with, is that right? Or do I need a different size needle for reconstitution?

3. Am I adding the water to the peptide the same way I draw and inject the solution? Like drawing air into the syringe, putting it in the water and drawing water, then injecting into the peptide vial and swirling? Is that right?

Thanks for any help!
 
Hey there, you're on the right path!

1.) Temp: Yeah, let them get close to room temperature. Water: maybe 5-10 minutes out of the fridge. Peptide: 20-30 minutes if frozen. This is to help it dissolve smoothly.

2.) Needles: Insulin syringes work well for the small vials. Go slow when you inject. Some use a bigger needle just for mixing if the peptides are thick, then switch back for injecting. I think you'll be good with what you have though.

3.) Water: Yes, you're right. Get the right amount of water. Inject it slowly down the side of the vial, not right onto the peptides.

Some tips:
* Wipe the tops of the vials with alcohol before you poke them, to keep things clean.
* Keep the vial upright when you're drawing the solution to inject.
* Label the vials with the date after you mix them, and keep them in the fridge if you aren't using them right away.

Good luck! I know it can be scary the first time, but you've got this!
 
I don't put my bac water in the fridge. I keep it in a drawer, so it's always at room temp.
 
Thanks! Melt_Update179 dumb question, but when you say keep the vials upright while drawing, do you mean flat on the counter with the needle above it drawing upwards? Or holding the vial straight up and down above the needle, drawing downwards? I've been doing it the second way lol
 
Yea, that's what I plan to do now. But the person who gave me my first peptides mixed it, and put the bac water in the same container, so I just stuck it all in the fridge lol. I have one vial of bac water and one vial of peptide in the fridge right now.
 
That makes sense and sounds easier than the way I've been doing it lol, especially if it's important to keep the vials straight up and down when drawing.
 
Especially Hospira BAC water. Pfizer says to keep it at room temp. For other brands, it's not as clear once it's been opened. People store it both ways.
 
I find it easiest to flip the vial over and barely poke the rubber stopper. Speeds things up.

If you're at the end and want every drop, inject a whole syringe of air so you're not fighting a vacuum.

I've seen stuff here, even peptideresearchinstitute.org, that says to put both peptides and BAC in the fridge an hour before mixing.

I keep my Hospira at room temperature, then pop it in the fridge an hour before. Once it's open, I keep it in the fridge. Might not matter.
 
FYI on 10ml vials… the stoppers can trap liquid between the stopper and vial. For those, I use a bottle opener to take off the crimp after mixing, draw the liquid with a 27ga x 1.5” luer lock needle on a 3ml syringe, take off the needle, put on a syringe filter, and inject into a sterile vial.
 
The Hospira web site says "Do not refrigerate", store at room temp in a dark spot because the alcohol can lose its effectiveness at lower temps.
 
I saw someone asking about how much bacteriostatic water to mix with their peptides. The easiest way is to add the same amount of water as the amount of peptide powder. For instance, if you have a 5mg vial, add 5mL of water.
 
I agree with RXPete. If you put 5ml in a 5mg vial, this will give you 1mg per ml. So if you wanted 300mcg, you would draw to the 0.3ml mark on your insulin syringe. Easy!
 
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