EmptyFridgeClub
Active member
I've been digging into the whole salt form situation with tirz, but it's kinda murky. Anyone else have thoughts on what I've found? Thinking about contacting some suppliers and running my own tests.
Basically, how can you tell if your tirz is base or salt? You can't, unless you use a specific ion chromatography test, which isn't standard. Most of the stuff at these prices is probably salt form. Need more testing to know which salt is being used (sodium, acetate, etc.).
PeptideTests says:
We use tirzepatide sodium as the reference for content.
Purity is just a sum of non-tirz peaks divided by the tirz peak.
You can't tell the salt form in solution without testing.
Janoshik says:
Purity tests use the base form, disregarding counterions.
Salt forms aren't discernible in solution without specific testing.
To identify the salt, use ion chromatography (or Fluorine NMR for TFA, EDX for Sodium, etc.). Janoshik offers this.
Should we worry about salt forms?
No studies compare salt form differences in bioavailability/efficacy/safety. Only the base form was used in trials.
Some say salt forms are the same once dissolved, because the counterions "pop off," leaving tirz base + the counterion (sodium, acetate, etc.).
Salt forms can change bioavailability, pH, or lipophilicity. Different salts can have different effects. Don't assume they're all equal.
Testing facilities use tirzepatide sodium as a standard, and it's cheap to make. So, most grey market stuff is likely sodium or acetate. When you reconstitute, you get:
Tirzepatide Sodium -> Tirzepatide base + Sodium, or
Tirzepatide Acetate -> Tirzepatide base + Acetic Acid.
Salt forms:
Sodium Salt: For osmotic balance. Safe in controlled amounts.
Acetate Salt: Biocompatible, common in pharmaceuticals and supplements. Typically safe.
Hydrochloride Salt: Increases solubility, may need pH adjustment.
Trifluoroacetate Salt: Used in peptide synthesis but may be toxic. Should be purified out.
Formate Salts: Less common, limited safety data. Use with caution.
More research is needed to understand the distribution of tirzepatide salt forms.
Basically, how can you tell if your tirz is base or salt? You can't, unless you use a specific ion chromatography test, which isn't standard. Most of the stuff at these prices is probably salt form. Need more testing to know which salt is being used (sodium, acetate, etc.).
PeptideTests says:
We use tirzepatide sodium as the reference for content.
Purity is just a sum of non-tirz peaks divided by the tirz peak.
You can't tell the salt form in solution without testing.
Janoshik says:
Purity tests use the base form, disregarding counterions.
Salt forms aren't discernible in solution without specific testing.
To identify the salt, use ion chromatography (or Fluorine NMR for TFA, EDX for Sodium, etc.). Janoshik offers this.
Should we worry about salt forms?
No studies compare salt form differences in bioavailability/efficacy/safety. Only the base form was used in trials.
Some say salt forms are the same once dissolved, because the counterions "pop off," leaving tirz base + the counterion (sodium, acetate, etc.).
Salt forms can change bioavailability, pH, or lipophilicity. Different salts can have different effects. Don't assume they're all equal.
Testing facilities use tirzepatide sodium as a standard, and it's cheap to make. So, most grey market stuff is likely sodium or acetate. When you reconstitute, you get:
Tirzepatide Sodium -> Tirzepatide base + Sodium, or
Tirzepatide Acetate -> Tirzepatide base + Acetic Acid.
Salt forms:
Sodium Salt: For osmotic balance. Safe in controlled amounts.
Acetate Salt: Biocompatible, common in pharmaceuticals and supplements. Typically safe.
Hydrochloride Salt: Increases solubility, may need pH adjustment.
Trifluoroacetate Salt: Used in peptide synthesis but may be toxic. Should be purified out.
Formate Salts: Less common, limited safety data. Use with caution.
More research is needed to understand the distribution of tirzepatide salt forms.
